Strain-based amino acid sequence alignment
1.Open IniFlu_1.0.xlsm (If the security alerts on the Message Bar, click Enable Macro)
2.Select "Sequence Data Processing" Tab → "FluCS" → Click "Input all standard viral nomenclature"
3.Next, click the menu "Input segment viral nomenclature" → a virus protein
4.Then click FluCS → a virus protein
Repeat step 3~4 until all virus proteins are imported.
1.Select "Sequence Grouping and Computing" Tab → "Calculate the mother group consensus"
2.Select "Sequence Data Processing" Tab → "Sequence Processing" → "Residue annotate" menu → a virus protein
3.Next, click the menu "Hide UTP" → a virus protein
Repeat step 2~3 until all virus proteins are processed.
(If some residues are hidden and you want to show, you can use mouse right-click menu to show the column.)
1.Select "Sequence Grouping and Computing" Tab → Click "Grouping filter"
2.Click the "▼" on your interest information or resides to make a group.
3.Click "Calculate the daughter group consensus" and type the group file name.
The file will be save in the C:\Flutures\FluConvert\Output folder.
4.Click "Create a substitution table (strains or %)" to calculate the frequency of amino acids occurring.