1.Install mEMBOSS-22.214.171.124-setup.exe in the absolute path C:\mEMBOSS 2.Install mafft-win.exe in the absolute path C:\mEMBOSS 3.Install FluConvert_v1-setup.exe in the absolute path C:\Flutures
1.Sequence fasta file (for example: H5N2.fasta) put into C:\Flutures\FluConvert\Input folder 2.Run C:\Flutures\FluConvert\FluConvert.exe 3.Type virus subtype H5N2, then type 1 4."Do you want to translate the sequences to amino acid?", type Y 5.The completed result will be in the C:\Flutures\FluConvert\Output folder
FluConvert regarding the selection of viral subtype, we have only tested H5N2, H7N9, and H1N1 at present.
If you have other subtype requirements, please contact us.